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A New Method for Accelerated Aging of Nanoparticles to Assess the Colloidal Stability of Albumin-Coated Magnetic Nanoparticles

Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Nikolaev, Boris;
ORCID
0000-0001-6151-3617
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Yakovleva, Ludmila;
ORCID
0000-0002-0239-7473
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Fedorov, Viacheslav;
ORCID
0000-0002-7357-1571
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Yudintceva, Natalia;
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Tarasova, Daria;
ORCID
0000-0001-6365-4405
Affiliation
Research Center of Neurology, 125367 Moscow, Russia;
Perepelitsa, Elizaveta;
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Dmitrieva, Anastasia;
ORCID
0000-0003-4665-3181
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Sulatsky, Maksim;
ORCID
0000-0001-7730-0931
Affiliation
Department of Chemical Engineering, National Institute of Technology, Warangal 506004, Telangana State, India;(S.S.);(S.H.S.)
Srinivasan, Sivaprakash;
ORCID
0000-0002-3201-6731
Affiliation
Department of Chemical Engineering, National Institute of Technology, Warangal 506004, Telangana State, India;(S.S.);(S.H.S.)
Sonawane, Shirish H.;
ORCID
0000-0001-5092-9545
Affiliation
Department of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India;(A.S.);(S.G.);(A.S.)
Srivastava, Anusha;
ORCID
0000-0003-2521-0170
Affiliation
Department of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India;(A.S.);(S.G.);(A.S.)
Gupta, Sharad;
ORCID
0000-0002-9596-2629
Affiliation
Department of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India;(A.S.);(S.G.);(A.S.)
Sonawane, Avinash;
Affiliation
Department of Radiation Oncology, Technische Universität München (TUM), Klinikum Rechts der Isar, 81675 Munich, Germany;
Combs, Stephanie E.;
ORCID
0000-0002-8539-2239
Affiliation
Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia;(B.N.);(L.Y.);(V.F.);(N.Y.);(D.T.);(A.D.);(M.S.)
Shevtsov, Maxim

The colloidal long-storage stability of nanosized drugs is a crucial factor for pharmacology, as they require much time for robust estimation. The application of bioavailable magnetic nanosuspensions in theranostics is limited by incomplete information about their colloidal stability in the internal media of human organisms. A method for the accelerated temperature stress “aging” of magnetic nanosized suspensions is proposed for the rapid assessment and prediction of the colloidal stability over time of nanosized iron oxide suspensions stabilized by albumin HSA. Colloidal stability is assessed using dynamic light scattering (DLS), fluorescence spectroscopy, electrophoresis, and ion monitoring methods during short- and long-term storage. Rapid assessment is achieved by short high-temperature (70 °C) processing of carboxymethyl-dextran-coated nanosol in the presence of albumin. The role of albumin in the sustained stability of superparamagnetic iron oxide particles (SPIONs) was studied under conditions mimicking blood plasma (pH = 7.4) and endolysosomal cell compartments (pH = 5.5). According to the fluorescence quenching and DLS data, colloidal stability is ensured by the formation of an HSA corona on carboxymethyl-dextran-coated SPIONs and their process of clustering. In the presence of albumin, the colloidal stability of nanoparticles is shown to increase from 80 to 121 days at a storage temperature of 8 °C The prognostic shelf life of magnetic nanosol is estimated by calculating the Van’t Hoff’s relation for the rate of chemical reactions. The validity of using the Van’t Hoff’s rule is confirmed by the agreement of the calculated activation energy at 8 °C and 70 °C. The developed method of the accelerated aging of nanoparticles can not only be employed for the estimation of the shelf life of magnetic nanoparticles coated with HSA in vitro but also for assessing the stability of SPIONs applied in vivo.

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Editor:
Dippong, Thomas
Published in:
Nanomaterials  
Vol. 15, No. 7 (21.03.2025)
Volume:
15
No.:
7
Date Issued:
21.03.2025
DOI:
10.3390/nano15070475
Language:
English
Type of Resource:
Text
Keywords:
metal oxide nanoparticles; superparamagnetic iron oxide nanoparticles; SPIONs; protein corona; nanoparticle aggregation; method of accelerated aging; nanoparticle stability
DDC:
610 Medizin und Gesundheit

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