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Immunity to Tick-Borne Encephalitis Virus NS3 Protein Induced with a Recombinant Modified Vaccinia Virus Ankara Fails to Afford Mice Protection against TBEV Infection

Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Kubinski, Mareike;
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Beicht, Jana;
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Gerlach, Thomas;
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Aregay, Amare;
ORCID
0000-0001-6535-3497
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Osterhaus, Albert D. M. E.;
Affiliation
Division of Virology, Institute for Infectious Diseases and Zoonoses, Ludwig Maximilian University Munich, Sonnenstraße 24, 85764 Oberschleißheim, Germany;(A.T.);
Tscherne, Alina;
Affiliation
Division of Virology, Institute for Infectious Diseases and Zoonoses, Ludwig Maximilian University Munich, Sonnenstraße 24, 85764 Oberschleißheim, Germany;(A.T.);
Sutter, Gerd;
ORCID
0000-0002-6631-2096
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Prajeeth, Chittappen Kandiyil;
ORCID
0000-0002-1921-4581
Affiliation
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany;(M.K.);(J.B.);(T.G.);(A.A.);(A.D.M.E.O.);(C.K.P.)
Rimmelzwaan, Guus F.

Tick-borne encephalitis (TBE) is a serious neurological disease caused by TBE virus (TBEV). Because antiviral treatment options are not available, vaccination is the key prophylactic measure against TBEV infections. Despite the availability of effective vaccines, cases of vaccination breakthrough infections have been reported. The multienzymatic non-structural protein 3 (NS3) of orthoflaviviruses plays an important role in polyprotein processing and virus replication. In the present study, we evaluated NS3 of TBEV as a potential vaccine target for the induction of protective immunity. To this end, a recombinant modified vaccinia virus Ankara that drives the expression of the TBEV NS3 gene (MVA-NS3) was constructed. MVA-NS3 was used to immunize C57BL/6 mice. It induced NS3-specific immune responses, in particular T cell responses, especially against the helicase domain of NS3. However, MVA-NS3-immunized mice were not protected from subsequent challenge infection with a lethal dose of the TBEV strain Neudoerfl, indicating that in contrast to immunity to prME and NS1, NS3-specific immunity is not an independent correlate of protection against TBEV in this mouse model.

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Editor:
Zhang, Jinyang; Xiao, Chaoting
Published in:
Vaccines  
Vol. 12, No. 1 (20.01.2024)
Volume:
12
No.:
1
Date Issued:
20.01.2024
DOI:
10.3390/vaccines12010105
Language:
English
Type of Resource:
Text
Keywords:
  1. TBEV
    Typ
    Schlagwort
  1. MVA
    Typ
    Schlagwort
  1. NS3
    Typ
    Schlagwort
  1. vaccination
    Typ
    Schlagwort
  1. antibodies
    Typ
    Schlagwort
  1. T cells
    Typ
    Schlagwort
DDC:
610 Medizin und Gesundheit

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