Feedback

Effect of Calcitriol and Vitamin D Receptor Modulator 2 on Recovery of Injured Skeletal Muscle in Wistar Rats

ORCID
0000-0001-5522-0092
Affiliation
Department of Orthopaedic Surgery, University of Wuerzburg, 97074 Wuerzburg, Germany
Stratos, Ioannis;
Affiliation
Department of Trauma, Hand and Reconstructive Surgery, University of Rostock, 18057 Rostock, Germany
Schleese, Svenja;
ORCID
0000-0002-3482-1881
Affiliation
Department of Trauma, Hand and Reconstructive Surgery, University of Rostock, 18057 Rostock, Germany
Rinas, Ingmar;
Affiliation
Institute for Experimental Surgery, University of Rostock, 18057 Rostock, Germany;
Vollmar, Brigitte;
ORCID
0000-0002-0536-8045
Affiliation
Department of Trauma, Hand and Reconstructive Surgery, University of Rostock, 18057 Rostock, Germany
Mittlmeier, Thomas

Muscle injuries often result in functional limitations due to insufficient healing. This study assessed the influence of calcitriol and vitamin D Receptor Modulator 2 (VDRM2) on muscle regeneration in male Wistar rats following open blunt muscle injury. The injured left soleus muscle of the rats was treated for the first four days after trauma with local injections of either calcitriol, VDRM2, or a 10% ethanol solution (control). Although muscle strength significantly decreased post-injury, all groups showed gradual improvement but did not achieve full recovery. By the 14th day, calcitriol-treated rats significantly outperformed the control group in the incomplete tetanic force, with VDRM2-treated rats showing muscle strength values that fell between the control and calcitriol groups. Similar trends were observed in complete tetanic contractions and were confirmed histologically via muscle cell width quantification. Additionally, histological analysis showed increased cellular turnover on the fourth postoperative day in the calcitriol group, as indicated by elevated cell proliferation rates and fewer apoptotic cells. VDRM2-treated animals showed only an increased proliferative activity on day 4 after injury. No noticeable differences between the groups for CAE-positive cells or visible muscle tissue area were found. In conclusion, predominantly calcitriol positively influenced post-trauma muscle recovery, where VDRM2 had substantially lower biological activity.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: © 2023 by the authors.

Use and reproduction: