Reduction of Hydrogen Peroxide by Human Mitochondrial Amidoxime Reducing Component Enzymes
The mitochondrial amidoxime reducing component (mARC) is a human molybdoenzyme known to catalyze the reduction of various N -oxygenated substrates. The physiological function of mARC enzymes, however, remains unknown. In this study, we examine the reduction of hydrogen peroxide (H 2 O 2 ) by the human mARC1 and mARC2 enzymes. Furthermore, we demonstrate an increased sensitivity toward H 2 O 2 for HEK-293T cells with an MTARC1 knockout, which implies a role of mARC enzymes in the cellular response to oxidative stress. H 2 O 2 is a reactive oxygen species (ROS) formed in all living cells involved in many physiological processes. Furthermore, H 2 O 2 constitutes the first mARC substrate without a nitrogen–oxygen bond, implying that mARC enzymes may have a substrate spectrum going beyond the previously examined N -oxygenated compounds.