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A Novel Strategy of US3 Codon De-Optimization for Construction of an Attenuated Pseudorabies Virus against High Virulent Chinese Pseudorabies Virus Variant

Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Xu, Mengwei;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Wang, Yiwei;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Liu, Yamei;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Chen, Saisai;
Affiliation
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Zhu, Laixu;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Tong, Ling;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Zheng, Yating;
Affiliation
Institut für Virologie, Freie Universität Berlin, 14163 Berlin, Germany
Osterrieder, Nikolaus;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Zhang, Chuanjian;
Affiliation
National Research Center of Engineering and Technology for Veterinary Biologicals, Institute of Veterinary Immunology and Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China(S.C.);(J.W.)
Wang, Jichun

In this study, we applied bacterial artificial chromosome (BAC) technology with PRV ΔTK/gE/gI as the base material to replace the first, central, and terminal segments of the US3 gene with codon-deoptimized fragments via two-step Red-mediated recombination in E. coli GS1783 cells. The three constructed BACs were co-transfected with gI and part of gE fragments carrying homologous sequences (gI+gE’), respectively, in swine testicular cells. These three recombinant viruses with US3 codon de-optimization ((PRV ΔTK&gE-US3deop−1 , PRV ΔTK&gE-US3deop−2 , and PRV ΔTK&gE-US3deop−3 ) were obtained and purified. These three recombinant viruses exhibited similar growth kinetics to the parental AH02LA strain, stably retained the deletion of TK and gE gene fragments, and stably inherited the recoded US3. Mice were inoculated intraperitoneally with the three recombinant viruses or control virus PRV ΔTK&gEAH02 at a 10 7.0 TCID 50 dose. Mice immunized with PRV ΔTK&gE-US3deop−1 did not develop clinical signs and had a decreased virus load and attenuated pathological changes in the lungs and brain compared to the control group. Moreover, immunized mice were challenged with 100 LD 50 of the AH02LA strain, and PRV ΔTK&gE-US3deop−1 provided similar protection to that of the control virus PRV ΔTK&gEAH02 . Finally, PRV ΔTK&gE-US3deop−1 was injected intramuscularly into 1-day-old PRV-negative piglets at a dose of 10 6.0 TCID 50 . Immunized piglets showed only slight temperature reactions and mild clinical signs. However, high levels of seroneutralizing antibody were produced at 14 and 21 days post-immunization. In addition, the immunization of PRV ΔTK&gE-US3deop−1 at a dose of 10 5.0 TCID 50 provided complete clinical protection and prevented virus shedding in piglets challenged by 10 6.5 TCID 50 of the PRV AH02LA variant at 1 week post immunization. Together, these findings suggest that PRV ΔTK&gE-US3deop−1 displays great potential as a vaccine candidate.

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