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Phytochemical, Morphological and Genetic Characterisation of Anacyclus pyrethrum var. depressus (Ball.) Maire and Anacyclus pyrethrum var. pyrethrum (L.) Link

ORCID
0000-0003-1622-4490
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
Jawhari, Fatima Zahra;
ORCID
0000-0002-8410-3212
Affiliation
Faculty of Sciences, Arab American University Palestine, Jenin P.O. Box 240, Palestine
Imtara, Hamada;
ORCID
0000-0002-1173-470X
Affiliation
African Genome Center, Mohammed VI Polytechnic University (UM6P), Lot 660, Hay Moulay Rachid, Ben Guerir 43150, Morocco;
Radouane, Nabil;
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
El Moussaoui, Abdelfattah;
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
Es-safi, Imane;
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
Amaghnouje, Amal;
Affiliation
Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11761, Saudi Arabia;
N. AlZain, Mashail;
Affiliation
Department of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, Germany;
Noman, Omer;
ORCID
0000-0002-7154-9151
Affiliation
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia;
Parvez, Mohammad Khalid;
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
Bousta, Dalila;
Affiliation
Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, Morocco;(A.E.M.);(I.E.-s.);(A.A.);(D.B.);(A.B.)
Bari, Amina

The present study is based on a multidisciplinary approach carried out for the first time on Anacyclus pyrethrum var. pyrethrum and Anacyclus pyrethrum var. depressus , two varieties from the endemic and endangered medicinal species listed in the IUCN red list, Anacyclus pyrethrum (L.) Link. Therefore, morphological, phytochemical, and genetic characterisations were carried out in the present work. Morphological characterisation was established based on 23 qualitative and quantitative characters describing the vegetative and floral parts. The phytochemical compounds were determined by UHPLC. Genetic characterisation of extracted DNA was subjected to PCR using two sets of universal primers, rbcL a-f/rbcL a-R and rpocL1-2/rpocL1-4, followed by sequencing analysis using the Sanger method. The results revealed a significant difference between the two varieties studied. Furthermore, phytochemical analysis of the studied extracts revealed a quantitative and qualitative variation in the chemical profile, as well as the presence of interesting compounds, including new compounds that have never been reported in A. pyrethrum . The phylogenetic analysis of the DNA sequences indicated a similarity percentage of 91%. Based on the morphological characterisation and congruence with the phytochemical characterisation and molecular data, we can confirm that A. pyrethrum var. pyrethrum and A. pyrethrum var. depressus represent two different taxa.

Category

Editor:
Taviano, Maria Fernanda; Acquaviva, Rosaria; Malfa, Giuseppe Antonio
Published in:
Molecules  
Vol. 28, No. 14 (13.07.2023)
Volume:
28
No.:
14
Date Issued:
13.07.2023
DOI:
10.3390/molecules28145378
Language:
English
Type of Resource:
Text
Keywords:
Anacyclus pyrethrum var. pyrethrum (L.) Link; Anacyclus pyrethrum var. depressus (Ball.) Maire; morphological characterisation; physicochemical characterisation; genetic characterisation
Subject of DNB:
610 Medical sciences Medicine
DDC:
615 Pharmakologie, Therapie

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