Feedback

GC-MS chemical profiling, antioxidant, anti-diabetic, and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. and Thonn.) C.D. Adams leaves: experimental and computational studies

Affiliation
Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL) ,Department of Biochemistry ,Bowen University ,Iwo ,Nigeria
Ojo, Oluwafemi Adeleke;
Affiliation
Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL) ,Department of Biochemistry ,Bowen University ,Iwo ,Nigeria
Ogunlakin, Akingbolabo Daniel;
Affiliation
Department of Biochemistry ,Bingham University ,Karu ,Nigeria
Gyebi, Gideon Ampoma;
Affiliation
Department of Pure and Applied Biology ,Bowen University ,Iwo ,Nigeria
Ayokunle, Damilare IyinKristi;
Affiliation
Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL) ,Department of Biochemistry ,Bowen University ,Iwo ,Nigeria
Odugbemi, Adeshina Isaiah;
Affiliation
Department of Anatomy ,Bowen University ,Iwo ,Nigeria
Babatunde, Dare Ezekiel;
Affiliation
Department of Microbiology ,Bowen University ,Iwo ,Nigeria
Ajayi-Odoko, Omolola Adenike;
Affiliation
Department of Biochemistry ,Landmark University ,Omu-Aran ,Nigeria
Iyobhebhe, Matthew;
Affiliation
Department of Pharmacognosy and Environmental Medicine ,University of Nigeria ,Nsukka ,Nigeria
Ezea, Samson Chukwuemeka;
Affiliation
Department of Physiology ,Afe Babalola University ,Ado-Ekiti ,Nigeria
Akintayo, Christopher Oloruntoba;
Affiliation
Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL) ,Department of Biochemistry ,Bowen University ,Iwo ,Nigeria
Ayeleso, Ademola;
Affiliation
Department of Biochemistry ,Ekiti State University ,Ado-Ekiti ,Nigeria
Ojo, Adebola Busola;
Affiliation
Department of Biochemistry ,Ekiti State University ,Ado-Ekiti ,Nigeria
Ojo, Omolara Olajumoke

Introduction: This study aimed to investigate the chemical profile of GC-MS, antioxidant, anti-diabetic, and anti-inflammatory activities of the ethyl acetate fraction of Spilanthes filicaulis leaves (EFSFL) via experimental and computational studies. Methods: After inducing oxidative damage with FeSO 4 , we treated the tissues with different concentrations of EFSFL. An in-vitro analysis of EFSFL was carried out to determine its potential for antioxidant, anti-diabetic, and anti-inflammatory activities. We also measured the levels of CAT, SOD, GSH, and MDA. Results and discussion: EFSFL exhibited anti-inflammatory properties through membrane stabilizing properties (IC 50 = 572.79 μg/ml), proteinase inhibition (IC 50 = 319.90 μg/ml), and inhibition of protein denaturation (IC 50 = 409.88 μg/ml). Furthermore, EFSFL inhibited α-amylase (IC 50 = 169.77 μg/ml), α-glucosidase (IC 50 = 293.12 μg/ml) and DPP-IV (IC 50 = 380.94 μg/ml) activities, respectively. Our results indicated that induction of tissue damage reduced the levels of GSH, SOD, and CAT activities, and increased MDA levels. However, EFSFL treatment restores these levels to near normal. GC-MS profiling shows that EFSFL contains 13 compounds, with piperine being the most abundant. In silico interaction of the phytoconstituents using molecular and ensembled-based docking revealed strong binding tendencies of two hit compounds to DPP IV (alpha-caryophyllene and piperine with a binding affinity of −7.8 and −7.8 Kcal/mol), α-glucosidase (alpha-caryophyllene and piperine with a binding affinity of −9.6 and −8.9 Kcal/mol), and to α-amylase (piperine and Benzocycloheptano[2,3,4-I,j]isoquinoline, 4,5,6,6a-tetrahydro-1,9-dihydroxy-2,10-dimethoxy-5-methyl with a binding affinity of −7.8 and −7.9 Kcal/mol), respectively. These compounds also presented druggable properties with favorable ADMET. Conclusively, the antioxidant, antidiabetic, and anti-inflammatory activities of EFSFL could be due to the presence of secondary metabolites.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: Copyright © 2023 Ojo, Ogunlakin, Gyebi, Ayokunle, Odugbemi, Babatunde, Ajayi-Odoko, Iyobhebhe, Ezea, Akintayo, Ayeleso, Ojo and Ojo.

Use and reproduction: