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Characterization of large volume subcutaneous injections using computed tomography imaging and simultaneous pressure measurements

Affiliation
Translational Imaging ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Purcell, Mona;
Affiliation
Device Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Babaee, Sahab;
Affiliation
Device Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Galluppi, Michael;
Affiliation
Device Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Cline, John;
Affiliation
Device Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Hu, Guangli;
Affiliation
Laboratory Animal Resource ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Petrescu, Ioan;
Affiliation
Laboratory Animal Resource ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Hughes, Jennifer;
Affiliation
Laboratory Animal Resource ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Allen, Meredith;
Affiliation
Translational Imaging ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Messina, Eric;
Affiliation
Device Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Persak, Steven;
Affiliation
Preclinical Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Krishnamachari, Yogita;
Affiliation
Preclinical Development ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Lay-Fortenbery, Ashley;
Affiliation
Translational Imaging ,Merck Research Laboratories ,Merck and Co., Inc. ,Rahway ,NJ ,United States
Miller, Corin O.

Many commercially available biologics, previously delivered only intravenously, are being re-formulated for subcutaneous delivery to improve patient access and compliance. However, due to inherent solubility limitations, large volume injections (more than 2 mL) are typically required. Different strategies are being explored to improve the tolerability of such injections, including the co-formulation with hyaluronidase and/or implementing different needle designs. While there have been separate reports of measuring injection forces and using imaging to track injection delivery and tissue response, there is no current set of methods to simultaneously characterize the injection delivery (bleb) and measure injection pressures. In this study we describe the development of Computed Tomography imaging methods in minipigs to characterize the morphology of the bleb following injection, along with inline pressure measurements to assess subcutaneous pressure during injection using two different injection volumes, 4.5 mL and 9 mL. We show that these parameters change with injection volume, and that inclusion of hyaluronidase in the injection increases bleb dispersion and reduces skin distention while also lowering the injection pressure. This method will likely be a valuable tool for assessing and comparing different injection delivery methods and formulations.

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License Holder: Copyright © 2023 Purcell, Babaee, Galluppi, Cline, Hu, Petrescu, Hughes, Allen, Messina, Persak, Krishnamachari, Lay-Fortenbery and Miller.

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