Feedback

Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway

Affiliation
Department of Physiology ,Biochemistry and Pharmacology ,College of Veterinary Medicine ,University of Baghdad ,Baghdad ,Iraq
Alghetaa, Hasan;
Affiliation
Department of Physiology ,Biochemistry and Pharmacology ,College of Veterinary Medicine ,University of Baghdad ,Baghdad ,Iraq
Mohammed, Amira;
Affiliation
Department of Pathology ,Microbiology and Immunology ,School of Medicine ,University of South Carolina ,Columbia ,SC ,United States
Singh, Narendra;
Affiliation
Department of Pathology ,Microbiology and Immunology ,School of Medicine ,University of South Carolina ,Columbia ,SC ,United States
Wilson, Kiesha;
Affiliation
Department of Environmental Health Sciences ,Arnold School of Public Health ,University of South Carolina ,Columbia ,SC ,United States
Cai, Goushuai;
Affiliation
Dan L. Duncan Cancer Center ,Advanced Technology Core ,Alkek Center for Molecular Discovery ,Baylor College of Medicine ,Houston ,TX ,United States
Putluri, Nagireddy;
Affiliation
Department of Pathology ,Microbiology and Immunology ,School of Medicine ,University of South Carolina ,Columbia ,SC ,United States
Nagarkatti, Mitzi;
Affiliation
Department of Pathology ,Microbiology and Immunology ,School of Medicine ,University of South Carolina ,Columbia ,SC ,United States
Nagarkatti, Prakash

Acute Respiratory Distress Syndrome (ARDS) is triggered by a variety of insults, such as bacterial and viral infections, including SARS-CoV-2, leading to high mortality. In the murine model of ARDS induced by Staphylococcal enterotoxin-B (SEB), our previous studies showed that while SEB triggered 100% mortality, treatment with Resveratrol (RES) completely prevented such mortality by attenuating inflammation in the lungs. In the current study, we investigated the metabolic profile of SEB-activated immune cells in the lungs following treatment with RES. RES-treated mice had higher expression of miR-100 in the lung mononuclear cells (MNCs), which targeted mTOR, leading to its decreased expression. Also, Single-cell RNA-seq (scRNA seq) unveiled the decreased expression of mTOR in a variety of immune cells in the lungs. There was also an increase in glycolytic and mitochondrial respiration in the cells from SEB + VEH group in comparison with SEB + RES group. Together these data suggested that RES alters the metabolic reprogramming of SEB-activated immune cells, through suppression of mTOR activation and its down- and upstream effects on energy metabolism. Also, miR-100 could serve as novel potential therapeutic molecule in the amelioration of ARDS.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: Copyright © 2023 Alghetaa, Mohammed, Singh, Wilson, Cai, Putluri, Nagarkatti and Nagarkatti.

Use and reproduction: