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Phytochemical Characterization and Biological Evaluation of Origanum vulgare L. Essential Oil Formulated as Polymeric Micelles Drug Delivery Systems

ORCID
0000-0002-9367-4770
Affiliation
Department of Pharmacognosy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Bora, Larisa;
Affiliation
Pharmazentrum Frankfurt/ZAFES, Institute of General Pharmacology and Toxicology, Hospital of the Goethe University, 60596 Frankfurt am Main, Germany
Burkard, Tobias;
Affiliation
Pharmazentrum Frankfurt/ZAFES, Institute of General Pharmacology and Toxicology, Hospital of the Goethe University, 60596 Frankfurt am Main, Germany
Juan, Martina Herrero San;
ORCID
0000-0002-4499-2510
Affiliation
Pharmazentrum Frankfurt/ZAFES, Institute of General Pharmacology and Toxicology, Hospital of the Goethe University, 60596 Frankfurt am Main, Germany
Radeke, Heinfried H.;
Affiliation
Department II—Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Muț, Ana Maria;
Affiliation
Department II—Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Vlaia, Lavinia Lia;
ORCID
0000-0002-2223-5228
Affiliation
Department of Pharmacognosy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Magyari-Pavel, Ioana Zinuca;
ORCID
0000-0002-5325-3859
Affiliation
Department of Food Science and Technology, Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine, Calea Manastur, 3-5, 400372 Cluj-Napoca, Romania
Diaconeasa, Zorița;
ORCID
0000-0003-0594-4628
Affiliation
Department of Food Science and Technology, Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine, Calea Manastur, 3-5, 400372 Cluj-Napoca, Romania
Socaci, Sonia;
ORCID
0000-0003-3406-0303
Affiliation
Department of Analytical Chemistry, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Borcan, Florin;
ORCID
0000-0001-7187-0239
Affiliation
Department of Pharmacognosy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Kis, Brigitta;
ORCID
0000-0001-9100-4530
Affiliation
Research Center for Pharmaco-Toxicological Evaluation, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Muntean, Delia;
Affiliation
Research Center for Pharmaco-Toxicological Evaluation, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Dehelean, Cristina Adriana;
ORCID
0000-0001-5999-1214
Affiliation
Department of Pharmacognosy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania
Danciu, Corina

This study presents phytochemical characterization and biological evaluation of Origanum vulgare L. essential oil (OEO) formulated as polymeric micelles drug delivery systems as a possible non-invasive approach for the management of skin tags. GC-MS analysis of Romanian OEO revealed the identification and quantification of 43 volatile compounds (thymol and carvacrol being the main ones). The antioxidant activity was shown by four consecrated methods: CUPRAC, ABTS, ORAC and DPPH. OEO was incorporated by micellar solubilization into a binary hydrogel based on a Pluronic F 127/L 31 block-copolymers mixture. The pH, consistency, spreadability, particle size, polydispersity index and zeta potential of the OEO-loaded poloxamer-based binary hydrogel (OEO-PbH) were investigated. OEO-PbH was skin compatible in terms of pH and exhibited adequate spreadability and consistency. The minimal inhibitory concentrations of the tested OEO were similar to those obtained for the formulation, lower (2.5 µg/mL) for yeast and higher (40–80 µg/mL) for Gram-negative bacilli. As keratinocytes are among main components of skin tags, an in vitro evaluation was conducted in order to see the effect of the formulation against HaCaT human keratinocytes. OEO-PbH decreased HaCaT cells migration and proliferation and elicited a cytotoxic and pro-apoptotic effect in a dose- and time-dependent manner. No harmful effect on the viability of dendritic cells (DCs) was detected following the incubation with different concentrations (0–200 µg/mL) of the 5% formulation. Treatment in inflammatory DCs (+LPS) indicated a decrease in cytokine production of IL-6, TNF-α and IL-23 but no significant effect on IL-10 in any of the tested concentrations.

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