Feedback

Daniellia oliveri (Rolfe) Hutch and Dalziel: Antimicrobial Activities, Cytotoxicity Evaluation, and Phytochemical Identification by GC-MS

ORCID
0000-0002-0350-6985
Affiliation
Department of Bioorganic Chemistry, School of Pharmacy, University of Saarland, Campus B2 1, D-66123 Saarbruecken, Germany
Tittikpina, Nassifatou Koko;
ORCID
0000-0001-8500-1755
Affiliation
CNRS, L2CM, Université de Lorraine, F-57000 Metz, France
Kirsch, Gilbert;
ORCID
0000-0003-4349-5595
Affiliation
CNRS, L2CM, Université de Lorraine, F-54000 Nancy, France
Duval, Raphaël Emmanuel;
ORCID
0000-0001-7588-8751
Affiliation
LCP-A2MC, Université de Lorraine, F-57000 Metz, France
Chaimbault, Patrick;
ORCID
0000-0002-6288-7220
Affiliation
Department of Bioorganic Chemistry, School of Pharmacy, University of Saarland, Campus B2 1, D-66123 Saarbruecken, Germany
Jacob, Claus

During a previous study that identified plants used in traditional medicine in Togo to treat infectious diseases, Daniellia oliveri was specifically reported to treat intertrigo and candidiasis. Consequently, to explore the anti-infective potential of this plant, we investigated the antibacterial and the antifungal activity of the plant’s parts, as well as the cytotoxic activities of raw extracts and subsequent fractions, and the chemical composition of the most active fractions. In order to evaluate the antimicrobial activity, MICs were determined using the broth dilution method. Then, the most active fractions were evaluated for cytotoxicity by using normal human cells (MRC-5 cells) via the MTT assay. Finally, the most active and not toxic fractions were phytochemically investigated by GC-MS. Interestingly, all the raw extracts and fractions were active against the bacteria tested, with MICs ranging from 16 µg/mL to 256 µg/mL, while no antifungal activity was observed at 256 µg/mL, the highest tested concentration. Moreover, no toxicity was observed with most of the active fractions. The subsequent chemical investigation of the most interesting fractions led to identifying terpenes, phytosterols, phenolic compounds, and fatty acids as the main compounds. In conclusion, this study demonstrated that D. oliveri possesses valuable antibacterial activities in accordance with traditional use.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: © 2022 by the authors.

Use and reproduction: