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Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats

Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Chen, Wanyu;
Affiliation
Shenzhen Hospital of Shanghai University of Traditional Chinese Medicine ,Guangzhou ,China
Liao, Lu;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Huang, Zitong;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Lu, Yulin;
Affiliation
College of Integrated Chinese and Western Medicines, Hunan University of Chinese Medicine ,Changsha ,Hunan ,China
Lin, Yukang;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Pei, Ying;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Yi, Shulin;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Huang, Chen;
Affiliation
School of Chinese Materia Medica ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Cao, Hongying;
Affiliation
Research Centre of Basic Intergrative Medicine ,School of Basic Medical Sciences ,Guangzhou University of Chinese Medicine ,Guangzhou ,China
Tan, Bo

Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba , has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown. Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses. Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats. Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D.

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License Holder: Copyright © 2022 Chen, Liao, Huang, Lu, Lin, Pei, Yi, Huang, Cao and Tan.

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