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CP-25 exerts a protective effect against ConA-induced hepatitis via regulating inflammation and immune response

Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Li, Nan;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Wu, Jing-Jing;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Qi, Meng;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Wang, Zi-Ying;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Zhang, Sheng-Nan;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Li, Xiu-Qin;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Chen, Ting-Ting;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Wang, Mei-Fang;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Zhang, Ling-Ling;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Wei, Wei;
Affiliation
Institute of Clinical Pharmacology ,Anhui Medical University ,Key Laboratory of Anti-inflammatory and Immune Medicine ,Ministry of Education ,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine ,Hefei ,China
Sun, Wu-Yi

Hepatitis is a complex multifactorial pathological disorder, which can eventually lead to liver failure and even potentially be life threatening. Paeoniflorin-6′-O-benzene sulfonate (CP-25) has proven to have critical anti-inflammatory effects in arthritis. However, the effects of CP-25 in the pathogenesis of hepatitis remains unclear. In this experiment, mice were intragastrically administered with CP-25 (25, 50 and 100 mg/kg), and then ConA (25 mg/kg) was intravenous injected to establish hepatitis model in vivo . CP-25 administration attenuated liver damage and decreased ALT and AST activities in mice with hepatitis. Besides, CP-25 modulated immune responses including down-regulated the proportions of activated CD4 + , activated CD8 + T cells, and ratio of Th1/Th2 in ConA-injected mice. Furthermore, ConA-mediated production of reactive oxygen species (ROS), release of inflammatory cytokines including IFN-γ, TNF-α, activation of MAPK pathways and nuclear translocation of nuclear factor-kappaB (NF-κB) were significantly decreased in CP-25 administrated mice. In ConA-stimulated RAW264.7 cells, CP-25 suppressed inflammatory cytokines secretion and reduced ROS level, which were consistent with animal experiments. Otherwise, the data showed that CP-25 restrained phosphorylation of ERK, JNK and p38 MAPK pathways influenced by ROS, accompanied with inhibiting NF-κB nuclear translocation. In conclusion, our findings indicated that CP-25 protected against ConA-induced hepatitis may through modulating immune responses and attenuating ROS-mediated inflammation via the MAPK/NF-κB signaling pathway.

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License Holder: Copyright © 2022 Li, Wu, Qi, Wang, Zhang, Li, Chen, Wang, Zhang, Wei and Sun.

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