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Ameliorative effect of Berberidis radix polysaccharide selenium nanoparticles against carbon tetrachloride induced oxidative stress and inflammation

Affiliation
College of Chemistry and Pharmaceutical Sciences ,Qingdao Agricultural University ,Qingdao ,China
Gao, Fei;
Affiliation
Agricultural Bio-Pharmaceutical Laboratory ,Qingdao Agricultural University ,Qingdao ,China
Liu, Huimin;
Affiliation
Agricultural Bio-Pharmaceutical Laboratory ,Qingdao Agricultural University ,Qingdao ,China
Han, Hao;
Affiliation
College of Veterinary Medicine ,Qingdao Agricultural University ,Qingdao ,China
Wang, Xin;
Affiliation
College of Chemistry and Pharmaceutical Sciences ,Qingdao Agricultural University ,Qingdao ,China
Qu, Lihua;
Affiliation
College of Chemistry and Pharmaceutical Sciences ,Qingdao Agricultural University ,Qingdao ,China
Liu, Congmin;
Affiliation
Shandong Provincial Key Laboratory of Applied Mycology ,Qingdao Agricultural University ,Qingdao ,China
Tian, Xuemei;
Affiliation
College of Chemistry and Pharmaceutical Sciences ,Qingdao Agricultural University ,Qingdao ,China
Hou, Ranran

Berberidis radix polysaccharide (BRP) extracted as capping agents was applied to prepare BRP-selenium nanoparticles (BRP-SeNPs) in the redox reaction system of sodium selenite and ascorbic acid. The stability and characterization of BRP-SeNPs were investigated by physical analysis method. The results revealed that BRP were tightly wrapped on the surface of SeNPs by forming C-O⋯Se bonds or hydrogen bonding interaction (O-H⋯Se). BRP-SeNPs presented irregular, fragmented and smooth surface morphology and polycrystalline nanoring structure, and its particle size was 89.4 nm in the optimal preparation condition. The pharmacologic functions of BRP-SeNPs were explored in vitro and in vivo . The results showed that BRP-SeNPs could heighten the cell viabilities and the enzyme activity of GSH-Px and decrease the content of MDA on H 2 O 2 -induced AML-12 cells injury model. In vivo tests, the results displayed that BRP-SeNPs could increase the body weight of mice, promote the enzyme activity like SOD and GSH-Px, decrease the liver organ index and the hepatic function index such as ALT, AST, CYP2E1, reduce the content of MDA, and relieve the proinflammation factors of NO, IL-1β and TNF-α in CCl 4 -induced mice injury model. Liver tissue histopathological studies corroborated the improvement of BRP-SeNPs on liver of CCl 4 -induced mice. The results of Western blot showed that BRP-SeNPs could attenuate oxidant stress by the Nrf2/Keap1/MKP1/JNK pathways, and downregulate the proinflammatory factors by TLR4/MAPK pathway. These findings suggested that BRP-SeNPs possess the hepatoprotection and have the potential to be a green liver-protecting and auxiliary liver inflammation drugs.

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License Holder: Copyright © 2022 Gao, Liu, Han, Wang, Qu, Liu, Tian and Hou.

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