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Production and Characterization of a Novel Exopolysaccharide from Ramlibacter tataouinensis

Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Jivkova, Desislava;
ORCID
0000-0001-5356-3438
Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Sathiyanarayanan, Ganesan;
Affiliation
Research Unit Analytical BioGeoChemistry (BGC), Helmholtz Munich, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
Harir, Mourad;
Affiliation
Research Unit Analytical BioGeoChemistry (BGC), Helmholtz Munich, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
Hertkorn, Norbert;
ORCID
0000-0003-0824-2664
Affiliation
Research Unit Analytical BioGeoChemistry (BGC), Helmholtz Munich, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
Schmitt-Kopplin, Philippe;
Affiliation
White Biotechnology Department, ARD, F-51110 Pomacle, France
Sanhaji, Ghislain;
Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Fochesato, Sylvain;
ORCID
0000-0001-8784-5396
Affiliation
CEA, CNRS, IPM, Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Berthomieu, Catherine;
Affiliation
CNRS, CERMAV, 38000 Grenoble, France
Heyraud, Alain;
ORCID
0000-0003-4297-6661
Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Achouak, Wafa;
Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Santaella, Catherine;
Affiliation
CEA, CNRS, Laboratory for Microbial Ecology (LEMiRE), Aix Marseille University, UMR7265 BIAM, F-13108 Saint-Paul-lez-Durance, France
Heulin, Thierry

The current study examines the desiccation-resistant Ramlibacter tataouinensis TTB310 T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid method. The biochemical characterization of purified exopolysaccharide was performed by gas chromatography, ultrahigh-resolution mass spectrometry, nuclear magnetic resonance, and Fourier-transform infrared spectrometry. The repeating unit of exopolysaccharide was a decasaccharide consisting of ribose, glucose, rhamnose, galactose, mannose, and glucuronic acid with the ratio 3:2:2:1:1:1, and additional substituents such as acetyl, succinyl, and methyl moieties were also observed as a part of the exopolysaccharide structure. This study contributes to a fundamental understanding of the novel structural features of exopolysaccharide from a dividing form of cysts, and, further, results can be used to study its rheological properties for various industrial applications.

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