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Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

Affiliation
Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, Germany
Hovhannisyan, Zaruhi;
Affiliation
Department of Medical Biochemistry and Biotechnology, Russian-Armenian University, 0051 Yerevan, Armenia
Timotina, Marina;
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Manoyan, Jemma;
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Gabrielyan, Lilit;
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Petrosyan, Margarit;
ORCID
0000-0001-8138-3872
Affiliation
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
Kusznierewicz, Barbara;
ORCID
0000-0002-6096-5838
Affiliation
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
Bartoszek, Agnieszka;
ORCID
0000-0002-6288-7220
Affiliation
Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, Germany
Jacob, Claus;
ORCID
0000-0002-8585-5539
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Ginovyan, Mikayel;
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Trchounian, Karen;
ORCID
0000-0002-7370-1380
Affiliation
Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
Sahakyan, Naira;
ORCID
0000-0003-2911-2607
Affiliation
Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, Germany
Nasim, Muhammad Jawad

Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant ( Ribes nigrum ) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, R. nigrum leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria ( Escherichia coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H + -fluxes in both strains of E. coli , even in the presence of N,N’ -dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria.

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