Feedback

Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approach

Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Shen, Yunfu;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Guo, Kaiqiang;
Affiliation
School of Biotechnology and Health Sciences ,Wuyi University ,Jiangmen ,China
Ma, Aijun;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Huang, Zhe;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Du, Jingjing;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Chen, Junhe;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Lin, Qianyu;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Wei, Chengming;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Wang, Zi;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Zhang, Fuming;
Affiliation
Antibody Engineering Laboratory ,School of Life Science & Technology ,China Pharmaceutical University ,Nanjing ,China
Zhang, Juan;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Lin, Wanjun;
Affiliation
School of Biotechnology and Health Sciences ,Wuyi University ,Jiangmen ,China
Feng, Na;
Affiliation
State Key Laboratory of Quality Research in Chinese Medicine ,Macau University of Science and Technology ,Macao ,Macao SAR ,China
Ma, Wenzhe

There are technical obstacles in the safety evaluation of traditional Chinese medicine (TCM) injections due to their complex chemical nature and the lack of rapid and accurate in vitro methods. Here, we established a dual in vitro mitochondrial toxicity approach combing the conventional “glucose/galactose” assay in HepG2 cells with the cytotoxic assay in mitochondrial respiration deficient cells. Using this dual in vitro approach, for the first time, we systematically assessed the mitochondrial toxicity of TCM injections. Four of the 35 TCM injections, including Xiyanping, Dengzhanhuasu, Shuanghuanglian, and Yinzhihuang, significantly reduced cellular ATP production in galactose medium in the first assay, and presented less cytotoxic in the respiration deficient cells in the second assay, indicating that they have mitochondrial toxicity. Furthermore, we identified scutellarin, rutin, phillyrin, and baicalin could be the potential mitochondrial toxic ingredients in the 4 TCM injections by combining molecular docking analysis with experimental validation. Collectively, the dual in vitro approach is worth applying to the safety evaluation of more TCM products, and mitochondrial toxic TCM injections and ingredients found in this study deserve more attention.

Graphical Abstract A dual in vitro mitochondrial toxicity assay approach combing the conventional “glucose/galactose” assay in HepG2 cells with the cytotoxic assay in mitochondrial respiration deficient cells was established in this study. Using this platform, we systematically assessed the mitochondrial toxicity of TCM injections for the first time. Four TCM injections were identified with potential mitochondrial toxicity. Their toxic ingredients were predicted by molecular docking and validated by the dual in vitro approach.

Cite

Citation style:
Could not load citation form.

Rights

License Holder: Copyright © 2022 Shen, Guo, Ma, Huang, Du, Chen, Lin, Wei, Wang, Zhang, Zhang, Lin, Feng and Ma.

Use and reproduction: