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Zuogui Wan ameliorates high glucose-induced podocyte apoptosis and improves diabetic nephropathy in db/db mice

Affiliation
Department of Nephrology ,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine ,Shanghai ,China
Zhu, Bingbing;
Affiliation
Department of Nephrology ,Putuo Hospital ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Fang, Ji;
Affiliation
Shanghai Key Laboratory of Complex Prescriptions and MOE Key Laboratory for Standardization of Chinese Medicines ,Institute of Chinese Materia Medica ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Ju, Zhengcai;
Affiliation
Department of Nephrology ,Putuo Hospital ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Chen, Ying;
Affiliation
Department of Nephrology ,Putuo Hospital ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Wang, Li;
Affiliation
Department of Nephrology ,Putuo Hospital ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Wang, Hao;
Affiliation
Department of Nephrology ,Putuo Hospital ,Shanghai University of Traditional Chinese Medicine ,Shanghai ,China
Xing, Lina;
Affiliation
Department of Nephrology ,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine ,Shanghai ,China
Cao, Aili

Zuogui Wan (ZGW), a well-known traditional Chinese medicine (TCM), has been used to nourish “Kidney-Yin” for a long time in China, implying a protective effect on the kidney. The aim of the present study is to investigate the effect of ZGW on high glucose-induced podocyte apoptosis and diabetic nephropathy (DN) in db/db mice. ZGW (1 g/kg −1 /day −1 ) was administered intragastrically to db/db mice for 8 weeks. HPLC was used for identifying the components of ZGW, biochemical and histopathological approaches were used for evaluating its therapeutic effects, and cultured mouse podocytes were used for further exploring its underlying mechanism in vitro. ZGW improved renal function and podocyte loss and also normalized kidney reactive oxygen species production in db/db mice. The cytotoxicity of ZGW on mouse podocytes was assessed by the LDH assay. The effect of ZGW on podocyte viability and apoptosis was determined with CCK-8 and Annexin-V/PI staining by treatment with high glucose. ZGW attenuated podocyte apoptosis, and oxidative stress was detected by the peroxide-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) staining in a dose-dependent manner. Furthermore, ZGW decreased the expression of caspase-3 and phospho-p38 in both the kidney cortex and high glucose-treated podocytes. Thus, our data from in vivo and in vitro studies demonstrated that ZGW improved renal injury in diabetes by inhibiting oxidative stress and podocyte apoptosis.

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License Holder: Copyright © 2022 Zhu, Fang, Ju, Chen, Wang, Wang, Xing and Cao.

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