Feedback

Production of Bovine Collagen Hydrolysate with Antioxidant Activity; Optimized by Response Surface Methodology

ORCID
0000-0003-1412-0890
Affiliation
Werner Siemens Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich (TUM), Lichtenberg Street 4, 85748 Garching bei München, Germany
Pakbin, Babak;
Affiliation
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin 34197-59811, Iran
Allahyari, Samaneh;
ORCID
0000-0002-4811-5312
Affiliation
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin 34197-59811, Iran
Dibazar, Shaghayegh Pishkhan;
ORCID
0000-0002-4516-5146
Affiliation
Institute for Life Technologies, University of Applied Sciences Western Switzerland Valais-Wallis, 1950 Sion 2, Switzerland
Brück, Wolfram Manuel;
Affiliation
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin 34197-59811, Iran
Vahidi, Roghayeh;
Affiliation
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin 34197-59811, Iran
Mahmoudi, Razzagh;
ORCID
0000-0001-6331-7628
Affiliation
Department of Food Hygiene and Quality of Control, Faculty of Veterinary Medicine, University of Tehran, Tehran 14199-63111, Iran
Khanjari, Ali

Antioxidants are widely used in pharmaceutical industries. Gelatin is a byproduct of the meat industry and its hydrolysates showed several functionalities, such as antioxidant activity. The purpose of this study was to describe and optimize the enzymatic hydrolysis conditions including time, temperature, pH, and enzyme/substrate ratio (E/S) to produce protein hydrolysate with antioxidant functionality from bovine gelatin by RSM; the scavenging activity was evaluated using the DPPH method. The model observed was fitted with desirable adequacy and sufficiency. We found that the antioxidant activity increased significantly ( p < 0.05) with the increase in pH value, E/S ratio, and time of enzymatic process; however, the temperature had no significant ( p < 0.05) effect on the antioxidant activity of the hydrolysate. The optimum hydrolysis conditions were observed at a temperature of 35.3 °C, pH of 8.0, and E/S ratio at 2.5 after 2 h hydrolysis by trypsin enzyme. The results showed that the hydrolysate under these conditions, optimized by RSM, could be more effective on antioxidant activity. Regarding the antioxidant potential, gelatin hydrolysate can be used as an antioxidant supplement in pharmaceutical industries.

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: © 2022 by the authors.

Use and reproduction: