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Structure and Dynamics of Human Chemokine CCL16—Implications for Biological Activity

Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Weiergräber, Oliver H.;
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Petrović, Dušan;
ORCID
0000-0001-8206-6377
Affiliation
Klinik für Dermatologie, Universitätsklinikum Düsseldorf, 40225 Düsseldorf, Germany
Kislat, Andreas;
Affiliation
Zentralinstitut für Engineering, Elektronik und Analytik, ZEA-3: Analytik, Forschungszentrum Jülich, 52425 Jülich, Germany
Pattky, Martin;
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Fabig, Judith;
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Batra-Safferling, Renu;
ORCID
0000-0002-2101-5012
Affiliation
Klinik für Allgemein-, Viszeral- und Kinderchirurgie, Universitätsklinikum Düsseldorf, 40225 Düsseldorf, Germany
Schulte am Esch, Jan;
ORCID
0000-0001-8314-9668
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Hänel, Karen;
ORCID
0000-0001-6865-1043
Affiliation
Zentralinstitut für Engineering, Elektronik und Analytik, ZEA-3: Analytik, Forschungszentrum Jülich, 52425 Jülich, Germany
Huhn, Carolin;
ORCID
0000-0002-8734-7765
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Strodel, Birgit;
Affiliation
Klinik für Dermatologie, Universitätsklinikum Düsseldorf, 40225 Düsseldorf, Germany
Homey, Bernhard;
ORCID
0000-0002-0065-7366
Affiliation
Institute of Biological Information Processing, IBI-7: Structural Biochemistry, Forschungszentrum Jülich, 52425 Jülich, Germany
Willbold, Dieter

Human C-C motif ligand 16 (CCL16) is a chemokine that is distinguished by a large cleavable C-terminal extension of unknown significance. Conflicting data have been reported concerning its tissue distribution and modulation of expression, rendering the biological function of CCL16 enigmatic. Here, we report an integrated approach to the characterisation of this chemokine, including a re-assessment of its expression characteristics as well as a biophysical investigation with respect to its structure and dynamics. Our data indicate that CCL16 is chiefly synthesised by hepatocytes, without an appreciable response to mediators of inflammation, and circulates in the blood as a full-length protein. While the crystal structure of CCL16 confirms the presence of a canonical chemokine domain, molecular dynamics simulations support the view that the C-terminal extension impairs the accessibility of the glycosaminoglycan binding sites and may thus serve as an intrinsic modulator of biological activity.

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